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Peer-Reviewed Publications   |   Clinical Guidelines & Books   |   Knowledge Dissemination   |   Reviews of Published Works 


DeMarco ML, Algeciras-Schimnich A, Budelier MM. Blood tests for Alzheimer’s disease: The Impact of Disease Prevalence on Test Performance. Alzheimer's & Dementia. 2024; available online ahead of print.

Patel K, Yang D, Feldman HH, Hsiung GYR, Nygaard H, Best JR, Dwosh E, Robillard JM, DeMarco ML. Personal value of Alzheimer’s disease biomarker testing and result disclosure from the patient and care partner perspective. Alzheimer's & Dementia. 2024;10(2):e12463.

Patel K, Yang D, Best JR, Chambers C, Lee PE, Henri-Bhargava A, Funnell CR, Foti DJ, Pettersen JA, Feldman HH, Nygaard HB, Hsiung GYR, DeMarco ML. Clinical value of Alzheimer’s disease biomarker testing. Alzheimer's & Dementia. 2024;10(2):e12464.


Feldman HH, Messer K, Qui Y, Sabbagh M, Galasko D, Turner RS, Lopez O, Smith A, Durrant J, Lupo J, Revta C, Balasubramanian A, Kuehn-Wache K, Wassmann T, Schell-Mader S, Jacobs DM, Salmon DP, Leger G, DeMarco ML, Weber F. Varoglutamstat: Inhibiting glutaminyl cyclase as a novel target of therapy in early Alzheimer’s disease. Journal of Alzheimer’s Disease. 2024; accepted.

Forgrave LM, Moon K, Hamden J, Li Y, Lu P, Foster LJ, Mackenzie IRA, DeMarco ML. Truncated TDP-43 proteoforms diagnostic of frontotemporal dementia with TDP-43 pathology. Alzheimer's & Dementia. 2024;20(1):103-111.


Duncan MC, … DeMarco ML, … Brumme ZL. Effects of COVID-19 mRNA vaccination on HIV viremia and reservoir size. AIDS 2024; in press.

Datwani S, … DeMarco ML, … Brockman MA. T cell responses to COVID-19 vaccines and breakthrough infection in people living with HIV receiving antiretroviral therapy. Viruses 2024;6(5): 661.

Cheung  PK, … DeMarco ML, … Brumme ZL. SARS-CoV-2 live virus neutralization after four COVID-19 vaccine doses in people with HIV receiving suppressive ART. AIDS. 2023;37(5):F11–F18.

Mwimanzi  F, … DeMarco ML, … Brumme ZL. Impact of age and SARS-CoV-2 breakthrough infection on humoral immune responses after three doses of COVID-19 mRNA vaccine. Open Forum Infectious Diseases. 2023;10(3):ofad073.

Chang A, Xiang X, Wang J, Lee C, Arakhamia T, Simjanoska M, Wang C, Carlomagno T, Zhang G, Dhingra S, Thierry M, Perneel J, Heeman B, Forgrave LM, DeTure M, DeMarco ML, Cook CN, Rademakers R, Dickson D, Petrucelli L, Stowell MHB, Mackenzie IRA, Fitzpatrick AWP. Homotypic fibrillization of TMEM106B across diverse neurodegenerative diseases. Cell. 2022;185(8):1346-1355.

Delaby C, … DeMarco ML, … Lehmann S. Clinical reporting following the quantification of cerebrospinal fluid biomarkers in Alzheimer's disease: An international overview. Alzheimer's & Dementia. 2022;18(10):18-79.

* Alzheimer's & Dementia journal Featured Article & among 2021 Top Downloaded Articles

Best JR, Wang M, Lee T, Russell JA, DeMarco ML. Early increases in anti-SARS-CoV-2 antibody isotypes associated with organ dysfunction and mortality in patients hospitalized with COVID-19. Intensive Care Medicine. 2022;48(5):616–618.


Lapointe H, ... DeMarco ML, ... Brumme ZL. Serial infection with SARS-CoV-2 Omicron BA.1 and BA.2 following three-dose COVID-19 vaccination. 2022;13:947021.

Lapointe H, ... DeMarco ML, ... Brumme ZL. People with HIV receiving suppressive antiretroviral therapy show typical antibody durability after dual COVID-19 vaccination, and strong third dose responses. The Journal of Infectious Diseases. 2022;jiac229.

Mwimanzi F, ... DeMarco ML, ... Brockman MA. Older Adults Mount Less Durable Humoral Responses to a Two-dose COVID-19 mRNA Vaccine Regimen, but Strong Initial Responses to a Third Dose. The Journal of Infectious Diseases. 2022;jiac199.

Brumme ZL, ... DeMarco ML, ... Brockman MA. Humoral immune responses to COVID-19 vaccination in people living with HIV receiving suppressive antiretroviral therapy. NPJ Vaccines. 2022;7(1):28.

Forgrave LM, van der Gugten JG, Nguyen Q, DeMarco ML. Establishing pre-analytical requirements and maximizing peptide recovery in the analytical phase for mass spectrometric quantification of amyloid-ß peptides 1-42 and 1-40 in CSF. Clinical Chemistry & Laboratory Medicine. 2022;60(2):198-206.


Brockman MA, Mwimanzi F, Lapointe HR, Sang Y, Agafitei O, Cheung P, Ennis S, Ng K, Basra S, Lim LY, Yaseen F, Young L, Umviligihozo G, Omondi FH, Kalikawe R, Burns L, Brumme CJ, Leung V, Montaner JSG, Holmes D, DeMarco ML, Simons J, Pantophlet R, Niikura M, Romney MG, Brumme ZL. Reduced magnitude and durability of humoral immune responses to COVID-19 mRNA vaccines among older adults. The Journal of Infectious Diseases. 2021;jiab592.

Forgrave LM, Wang M, Yang D, DeMarco ML. Proteoforms and their expanding role in laboratory medicine. Practical Laboratory Medicine. 2021;e00260.


Sherwood KR, Nicholl D, Fenninger F, Wu V, Wong P, Benedicto V, Davide CP, Wang M, Pobran TD, DeMarco ML, Marquez C, Jassem A, Sekirov I, Morshed M, Bardi M, Sekhon M, Keown P, Madatz M, James LH. Comprehensive immune profiling of a kidney transplant recipient with peri-operative SARS-CoV-2 infection: A case report. Frontiers Immunology. 2021;12:3834.

Quantitative Measurement of Proteins and Peptides by Mass Spectrometry. 1st ed. CLSI guideline C64. Clinical and Laboratory Standards Institute; 2021; ISBN 978-1-68440-110-9.


Pobran TD, Yang D, Mackenzie IRA, DeMarco ML. Aptamer-based enrichment of TDP-43 from human cells and tissues with quantification by HPLC-MS/MS. Journal of Neuroscience Methods. 2021;363:109344.

*Journal special edition: Methods and Models for Detection, Monitoring and Intervention of Neurological Disorders 

Gauiran DTV, Marcon KM, DeMarco ML, Fung AWS, van der Gugten JC, Mattman A, Carruthers MN, Song KY, Chen LYC. IgG4 plasma cell myeloma without clinical evidence of IgG4 related disease: a report of two cases. Hematology. 2020;25(1):335-340.

Multi-author publication*... DeMarco ML (contributing author). SARS-CoV-2 (COVID-19) Serology: A Review and Assessment of Potential Implications for Clinical Practice, Laboratory Medicine, and Public Health. Canadian Medical Association Journal. 2020;192(34):E973-E979.

*This is a collaborative multi-author publication by representatives of the Canadian Public Health Laboratory Network, Canadian Society of Clinical Chemists, Association of Medical Microbiology and Infectious Disease Canada, Canadian Association for Clinical Microbiology and Infectious Diseases, and Canada’s COVID-19 Immunity Task Force.


Funnell, C, Mackenzie IRA, Feldman HH, DeMarco ML. Applying the Alzheimer disease ATN diagnostic framework in atypical dementia. Alzheimer Disease & Associated Disorders. 2020;34:357-359.

Holmes DT, Romney M, Angel P, DeMarco ML. Proteomics Applications in Laboratory Medicine & Pathology: Present State and Future Prospects. Clinical Biochemistry. 2020;82:12-20.

Mattman A, Gilfix BM, Chen SX, DeMarco ML, Kyle BD, Parker ML, Agbor TA, Jung B, Selvarajah S, Barakauskas VE, Vaags AK, Estey MP, Nelson TN, Speevak MD. Alpha-1-antitrypsin molecular testing in Canada: A seven year, multi-centre comparison. Clinical Biochemistry. 2020;81:27-33.

DeMarco ML, Nguyen Q, Fok A, Hsiung GY, van der Gugten JG. An automated clinical mass spectrometric method for identification and quantification of variant and wild-type amyloid-beta peptides 1-40 and 1-42 in CSF. Alzheimer's & Dementia. 2020;e12036.

Singh S, Khayachi A, Milnerwood AJ, DeMarco ML. Quantitative profiling of synuclein species: Applications to transgenic mouse models of Parkinson's disease. Journal of Parkinson's Disease. 2020;10(2):614-621.

Mattman A, Chen SX, DeMarco ML, Estey MP, Kyle BD, Parker ML, Agbor TA, Kawada P, Speevak MD, Nelson TN. Null(Canada): A novel 1-antitrypsin allele in cis variants Glu366Lys and Ile100Asn. Clinical Biochemistry. 2020;79:23-27.

Singh S & DeMarco ML. In vitro conversion assays diagnostic for neurodegenerative proteinopathies. Journal of Applied Laboratory Medicine. 2020;5(1):142-157.
* Special collection: Brain Health: Biomarkers of Injury and Neurodegeneration

Forgrave LM, Ma M, Best JR, DeMarco ML. The diagnostic performance of neurofilament light chain in CSF and blood for Alzheimer’s disease, frontotemporal dementia, and amyotrophic lateral sclerosis: A systematic review and meta-analysis. Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring. 2019;11:730-743.

Shi J, Dhaliwal P, Zheng YZ, Wong T, Straseski JA, Cervinski MA, Shajani-Yi Z, DeMarco ML. An Intact ACTH LC-MS/MS Assay as an Arbiter of Clinically Discordant Immunoassay Results. Clinical Chemistry. 2019;65(11): 1397-1404.

* Clin Chem podcast; Clin Chem audio summary; Endocrine News article


Pobran TD, Forgrave LM, Zheng YZ, Lim JGK, Mackenzie IRA, DeMarco ML. Detection and Characterization of TDP-43 in Human Cells and Tissues by Multiple Reaction Monitoring Mass Spectrometry. Clinical Mass Spectrometry. 2019;14:66-73.
* Special issue: Clinical Mass Spectrometry Focusing on the Brain

Fung A, Kolhagen MC, DeMarco ML, Mills JR. A Rapidly Deteriorating Patient with Gross Elevation of Serum Free Light Chains. Clinical Chemistry. 2019;65(9):1084-1087.
* Associated commentaries by CR McCudden and by X Bossuyt & L Ancheva

Duncan M & DeMarco ML. MALDI-MS: Emerging Roles in Pathology and Laboratory Medicine. Clinical Mass Spectrometry. 2019;13:1-4.


van der Gugten JG, Bressler B, and DeMarco ML. An Automated Mass Spectrometric Blood Test for Therapeutic Drug Monitoring of Infliximab. Clinical Mass Spectrometry. 2019;12:16-22.

Speevak M, DeMarco ML, Wiebe N and Chapman K. An unusual case of alpha-1-antitrypsin deficiency: SZ/Z. Clinical Biochemistry. 2019;64:49-52.

Alotaibi NM, Chen V, Hollander Z, Leipsic JA, Hague CJ, Murphy D, DeMarco ML, FitzGerald JM, McManus BM, Ng RT, Sin DD. Phenotyping and Outcomes of Hospitalized COPD Patients Using Rapid Molecular Diagnostics on Sputum Samples. International Journal of COPD. 2019;14:311-319.

Shi J, Zheng YZ, Sin DD, DeMarco ML. A Streamlined Method for Quantification of Apolipoprotein A1 in Human Plasma by LC-MS/MS. Clinical Chemistry. 2018;64(12):1782-1784.

van der Gugten JG, DeMarco ML, Chen L, Chin A, Caruthers M, Holmes DT, Mattman A. Resolution of Spurious Immunonephelometric IgG Subclass Measurement Discrepancies by LC-MS/MS. Clinical Chemistry, 2018;64(4):735-742.
* This work resulted in remediation of an in vitro diagnostic device

Alotaibi NM, Chen V, Hollander Z, Leipsic JA, Hague CJ, Murphy D, DeMarco ML, FitzGerald JM, McManus BM, Ng RT, Sin DD. Phenotyping COPD Exacerbations Using Imaging and Blood-based Biomarkers. International Journal of COPD, 2018;12:217-229.

DeMarco ML. Amplification of misfolded prion proteins in blood and cerebrospinal fluid for detection of Creutzfeldt-Jakob disease. Clinical Chemistry, 2017;63(11):1671-1673. PMID:  28724625

Zheng YZ and DeMarco ML. Manipulating trypsin digestion conditions to accelerate proteolysis and simplify digestion workflows in the development of protein mass spectrometric assays for the clinical laboratory. Clinical Mass Spectrometry. 2017;6:1-12.

Chen Y-WR, Chen V, Hollander Z, Leung JM, Leipsic JA, Hague C, Murphy DT, DeMarco ML, FitzGerald JM, McManus BM, Ng RT, Sin DD. C-reactive protein and N-terminal prohormone brain natriuretic peptide as biomarkers to diagnose acute exacerbations of COPD leading to hospitalizations. PLoS ONE, 12(3): e0174063, 2017. PMID: 28328968

Hollander Z, DeMarco ML, Sadatsafavi M, McManus BM, Ng RT, Sin DD. Biomarker development in COPD: Moving from p-values to products to impact patient care. Chest. 2017:151(2):455-467. PMID: 27693595

Mitchell RA, Shuster C, Shahidi N, Galorport C, DeMarco ML, Rosenfeld G, Enns RA and Bressler B. The Utility of Infliximab Therapeutic Drug Monitoring Among Patients with Inflammatory Bowel Disease and Concerns for Loss of Response: A Retrospective Analysis of a Real-World Experience. Canadian Journal of Gastroenterology and Hepatology, Volume 2016, Article ID 5203898, 2016. PMID 27957480

Mattman A, DeMarco ML, Wong S, Holmes DT, Lee J. Grave clinicopathologic correlation: A case of hyperthyroxinemia. Journal of Applied Laboratory Medicine, 1(3):310-4, 2016. [PDF]
* Reviewed in JALM

Trasolini R and DeMarco ML. Therapeutic drug monitoring of monoclonal antibody infliximab. ASCP Case Reports, October, 2016.

Sin DD, Hollander Z, DeMarco ML, McManus BM and Ng RT. Biomarker development for COPD: From discovery to clinical implementation. American Journal of Respiratory and Critical Care Medicine, 192(10):1162-70, 2015. PMID 26176936

DeMarco ML, Dietzen DJ and Brown SM. Sweating the small stuff: Adequacy and accuracy in sweat chloride determination. Clinical Biochemistry, 48(6):443-447, 2015. [PDF]
* Reviewed in Clinical Laboratory News Stat

Hutton HL, DeMarco ML, Magil AB and Taylor P. Renal leucocyte chemotactic factor 2 (LECT2) amyloidosis in First Nations people in Northern British Columbia, Canada: A case series. American Journal of Kidney Diseases, 64:790-2, 2014. [PDF]

DeMarco ML and Burnham CAD. Diafiltration-MALDI-TOF Mass Spectrometry Method for Culture-Independent Detection and Identification of Pathogens Directly from Urine Specimens. American Journal of Clinical Pathology, 141:204-212, 2014. [PDF]

DeMarco ML. The Three-Dimensional Structure of Glycolipids in Biological Membranes. Biochemistry, 51(29):5725-32, 2012.

DeMarco ML and Woods RJ. From Agonist to Antagonist: Structure and Dynamics of Innate Immune Glycoprotein MD-2 upon Recognition of Variably Acylated Bacterial Endotoxins. Molecular Immunology. 2011;49:124-133.

DeMarco ML, Woods RJ, Prestegard JH and Tian F. Presentation of Membrane-Anchored Glycosphingolipids Determined from Molecular Dynamics Simulations and NMR Paramagnetic Relaxation Rate Enhancement. Journal of the American Chemical Society, 132:1334-1338, 2010.

DeMarco ML and Daggett V. Characterization of cell-surface prion protein relative to its recombinant analogue: Insights from molecular dynamics simulations of diglycosylated, membrane-bound human prion protein. Journal of Neurochemistry, 109:60-73, 2009.

DeMarco ML and Woods RJ. Atomic-resolution conformational analysis of GM3 ganglioside in a lipid bilayer, and its implications for ganglioside-protein recognition at membrane surfaces. Glycobiology, 19, 344-355, 2009.
* Selected for 2010 journal cover image

DeMarco ML and Woods RJ. Structural Glycobiology: A Game of Snakes and Ladders. Glycobiology (invited review), 18, 426-440, 2008.

Tessier MB, DeMarco ML, Yongye AB and Woods RJ. Extension of the GLYCAM06 Biomolecular Force Field to Lipids, Lipid Bilayers and Glycolipids. Molecular Simulation (Special Edition: Molecular Modelling of Carbohydrates), 34, 349-364, 2008.

DeMarco ML and Daggett, V. Molecular Mechanism for Low pH-Triggered Misfolding of the Human Prion Protein. Biochemistry, 46, 3045-3054, 2007.
* Selected as a Biochemistry "Hot Article" for 2007

DeMarco ML, Silveira J, Caughey B and Daggett V. Structural Properties of Prion Protofibrils and Fibrils: An Experimental Assessment of Atomic Models. Biochemistry, 45, 15573-15582, 2006.

DeMarco ML and Daggett V. Local environmental effects on the structure of the prion protein. Comptes Rendus Biologies (invited review), 328, 847-862, 2005.

DeMarco ML and Daggett V. From Conversion to Aggregation: Protofibril Formation of the Prion Protein. Proceedings of the National Academy of Sciences, USA, 101, 2293-2298, 2004.

Armen RS, DeMarco ML, Alonso DOV and Daggett V. Pauling and Corey’s α-pleated sheet structure may define the prefibrillar amyloidogenic intermediate in amyloid disease. Proceedings of the National Academy of Sciences, USA, 101, 11622-11627, 2004.
* Reviewed in Science and Nature

Bennion BJ, DeMarco ML and Daggett, V. Preventing misfolding of the prion protein by trimethylamine N-oxide. Biochemistry, 43, 12955-12963, 2004.

DeMarco ML, Alonso DOV and Daggett V. Diffusing and colliding: The atomic level folding/unfolding pathway of a small helical protein. Journal of Molecular Biology, 341, 1109-1124, 2004.

Gianni S, Guydosh NR, Khan F, Caldas TD, Mayor U, White GWN, DeMarco ML, Daggett V and Fersht AR. Unifying features in protein-folding mechanisms. Proceedings of the National Academy of Sciences, USA, 100, 13286-13291, 2003.

Preprint Publications

Lapointe  H, ... DeMarco ML, ... Brumme ZL. Antibody response durability following three-dose COVID-19 vaccination in people with HIV receiving suppressive ART. medRxiv. November 5, 2022

Lapointe H, ... DeMarco ML, ... Brumme ZL. Serial infection with SARS-CoV-2 Omicron BA.1 and BA.2 following three-dose COVID-19 vaccination. medRxiv  2022.05.19.22275026.

Lapointe H, ... DeMarco ML, ... Brumme ZL. People with HIV receiving suppressive antiretroviral therapy show typical antibody durability after dual COVID-19 vaccination, and strong third dose responses. medRxiv. March 23, 2022.


Mwimanzi F, ... DeMarco ML, ... Brockman MA. Older Adults Mount Less Durable Humoral Responses to a Two-dose COVID-19 mRNA Vaccine Regimen, but Strong Initial Responses to a Third Dose. medRxiv 2022.01.06.22268745.


Brumme ZL, ... DeMarco ML, ... Brockman MA. Humoral immune responses to COVID-19 vaccination in people living with HIV on
suppressive antiretroviral therapy
. medRxiv. October 15, 2021.

Brockman MA, … DeMarco ML, … Brumme ZL. Reduced magnitude and durability of humoral immune responses by COVID-19 mRNA vaccines among older adults. medRxiv 2021.09.06.21263149.

Brockman MA, ... DeMarco ML, ... Brumme ZL. Weak humoral immune reactivity among residents of long-term care facilities following one dose of the BNT162b2 mRNA COVID-19 vaccine. medRxiv. March 24, 2021

Clinical Guidelines & Book Chapters

CLSI guideline C62. Clinical and Laboratory Standards Institute; in development.


CLSI guideline C64. Clinical and Laboratory Standards Institute; 2021; ISBN 978-1-68440-110-9.


DeMarco ML and Gronowski AM. Reproductive Endocrine Function, in Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics (7th Edition), C. Burtis and D. Bruns Eds, Elsevier Inc, 2015. ISBN: 978-1-4557-4165-6


Molecular Dynamics Simulations of Membrane- and Protein-Bound Glycolipids Using GLYCAM.

DeMarco ML. Glycoinformatics, Methods in Molecular Biology, vol 1273, Springer, New York, 2015. ISBN: 978-1-4939-2343-3


DeMarco ML and Ford BA. Clinics in Laboratory Medicine (Issue: Automation and Emerging Technology in Clinical Microbiology), 33:611-628, 2013.

Knowledge Dissemination & Scientific Artwork
Image by Levi XU

Helbling CV, DeMarco ML. Eye Catching Advancement for Creutzfeldt-Jakob Disease Diagnostics. Clinical Chemistry. 2023;hvad154.


Farnsworth C, Zhao Z, Apple F, Collinson P, DeMarco ML, Gronowski A, Sacks D, Zhu Y. Critical roles of clinical laboratorians in translational research and applied sciences — experts’ insights and experiences. Clinical Chemistry. 2022;68(7):877–883.

Brain Scans

Shajani-Yi Z & DeMarco ML. Plasma ACTH: Tales of Diagnostic Misadventure and the Path Forward. Clinical Laboratory News, January 1, 2021.


Foster LJ & DeMarco ML. At the intersection of proteomics and big data science. Clinical Chemistry, 2017:63(10), 1663.


Lowe C & DeMarco ML. Ready, Set, Type! Proteomics vs Agglutination for E. coli H Antigen Confirmation. Clinical Chemistry, 2016:62(6):793-5.


DeMarco ML. The Lab and CJD: Safe Handling of Infectious Prion Proteins. Clinical Laboratory News, Bench Matters column. November 2015


Clinical Laboratory News - Daily Editions 2014 - Columns by Mari L. DeMarco:

Finding a needle in a haystack: Can lipids predict the onset of dementia? | Guidelines versus practice: Bridging the great divide in lipid and lipoprotein testing | Teaching an old dog new tricks: Developments in clinical PCR technology. | Move over warfarin, there are new oral anticoagulants in town


American Society for Biochemistry and Molecular Biology Today, "Gregarious Gangliosides", by Ronald L. Schnaar, September 2010


Glycobiology - Journal cover image for 2010


The Wellcome Trust's 'Big Picture', Epidemics, September 2007.


Chemical & Engineering News, Proteins From Birth to Death, by Stu Borman, Vol. 85, No. 35, Aug. 27, 2007.


University of Washington Research Calendar, 2006.


The Scientist, Unraveling Protein Folding by Melissa Lee Phillips, Vol. 19, Issue 7, 2005.

Articles About the Lab's Published Works
Alzheimer’s biomarkers: CSF reports, plasma prospects

CAP Today, by Anne Paxton, May 2023

Doctor Examining CT Scan
Trust but Verify: The Impact of Erroneous ACTH Assays

Endocrine News; January 2020


CLN Stat, January 2, 2020.


Journal of Applied Laboratory Medicine, January 22, 2015.

A Closer Look at Sweat Chloride Testing

Clinical Laboratory News - Stat, January 22, 2015.

New role for Pauling's ribbons

Nature, Christopher Surridge, 430, 739, 2004.


Science, Orla Smith, 305, 1534, 2004.

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